APPLICATION OF MARKER ASSISTED BACKCROSSING TO SELECT THE INDIVIDUAL PLANTS OF BC3F1 POPULATION CARRYING THE QTL/GENE TO IMPROVE YIELD OF KD18
Abstract
Rice (Oryza savita L.) is the most important food crop, and staple food for many people in the world. However, the pressure of rapid population growth, adverse effects from climate change and limited areas of rice growing in the country due to the urbanization and industrialization that need to urgently enhance rice yield to meet the increasing demands of food – consuming. Molecular breeding such as Marker – Assisted Backcrossing (MABC) is one of the efficient methods to transfer the specific quantitative trait loci (QTL) or gene into the elite varieties. In this study, MABC was applied to transfer QTL/gene which is responsible for trait of increasing number of grains per panicle from donor (KC25) to recipient plant (KD18). The results have shown that the successfully selected individual No 14 in BC3F1 population carrying QTL/gene and attained the highest genetic background of the recipient plant up to 100% were made.
References
Ashikari, M., Sakakibara, H., Lin, S., Yamamoto, T., Takashi, T., Nishimura, A., et al., Cytokinin oxidase regulates rice grain production. Science 309, 741–745, 2005.
Anh N.T.T, Trung T, Trung K H, Khanh T.D, Application of molecular breeding to select the individual plants of BC2F1 population carrying the QTL/Gene (increasing grains number per panicle) to improve yield of KD18 variety. Journal of Viet Nam Agricultural Science and Technology, 79(6), pp.3-7, 2017.
Frisch, M., M. Bohn, ADN A.E. Melchinger. Minimum sample size ADN optimal positioning of flanking markers in marker-assisted backcrossing for transfer of a target gene. Crop Sci, 39: 967-975, 1999.
Linh L.H, Hang N. T., Kang K.H, Lee Y.T, Kwon S.J, Ahn S.N, Introgression of a quantitative trait locut for spikelets per panicle from Oryza minuta to the O. sativa cultivar Hwaseongbyeo, Plant Bred 127, 262-267, 2008.
Li Y, Tao H, Zhao X, Xu J et al, Molecular Improvement of Grain Weight and Yield in Rice by Using GW6 Gene, Rice Science, 21(3): 127 – 132, 2014.
Noraziyah A. A.S et al, Marker assisted pyramiding of drought yield QTLs into a popular Malaysian rice cultivar, MR219, BMC Genetics, 2016.
